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A revised medium for rapid growth and bioassays with tobacco tissue cultures' by the University of Pennsylvania
Metalloproteases and Trypsin like Proteases
and permeating solutions that contain NP-40
Hygromycin B is often used for selection of transformants for gene transfer experimentation
EMM-Sodium Phosphate Size:250 g A revised medium for rapidEMM Sodium Phosphate Medium, 29. 57 g per liter CONTENTS: Potassium phthalate (3 g L) NH4Cl (5 g L) Dextrose (20 g L) SALTS: MgCl2 (anhydrous) (0. 492 g L) CaCl2 (14. 7 mg L) KCl (1 g L) Na2SO4 (0. 04 g L) VITAMINS: Pantothenic acid (1 mg L) Nicotinic acid (10 mg L) Inositol (10 mg L) D Biotin (0. 01 mg L) MINERALS: Boric acid (0. 5 mg L) MnSO4 (0. 4 mg L) ZnSO4 (0. 4 mg L) FeCl2 (0. 2 mg L) Molybdic acid (40 g L) Potassium Iodide (0. 1 mg L) CuSO4