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Vortex vigorously for at least 15 minutes before use — incomplete reconstitution is one of the most common sources of false results in BET workflows
red blood cells account for 55% of the cell suspension
② If the supernatant has been aspirated
increase the centrifugation speed from 300×g to 350×g and extend the centrifugation time to 8 minutes to enhance neuron precipitation
Bst DNA Polymerase, Full Length RNA Extraction Vortex vigorously for at leastBst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus that contains the 5' → 3' polymerase activity and the 5' → 3' exonuclease activity. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification. Applications Isothermal amplification (LAMP, RCA) DNA strand displacement amplification Primer extension Unit Definition One unit is
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