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and HRP conjugate are sequentially added to microwells pre-coated with a 90kDa heat shock protein alphaA1 (HSP90αA1) capture antibody
put it in the incubator for more than half an hour
Inflammation primarily stimulates monocytes
It is best to control the sample addition time within 5 minutes
E tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-911-14) Kits and HRP conjugate are sequentiallyProduct Specification Host Rabbit Antigen E tag Immunogen Synthetic Peptide Clone Number S 911 14 Antibody Type Recombinant mAb Isotype IgG Application ICC Reactivity Species Independent Purification Protein A Concentration 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied Dilution application dilution