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The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 6 in the sample
you can discard the circle around the plate and add PBS
add 100 μl of 3D Reagent to 100 μl of cell-containing culture medium)
Expression System HEK293 Molecular Weight 28-33kDa (Reducing) Purity >95% by SDS-PAGE Endotoxin <0
Alexa Fluor® 647 Mouse Anti-Human CD45 Antibody (S-839-3) Organoid Kit The A405 of the sampleProduct Specification Host Mouse Antigen CD45 Synonyms Receptor type tyrosine protein phosphatase C, Leukocyte common antigen (L CA), T200, PTPRC Immunogen Recombinant Protein Location Cell membrane Accession P08575 Clone Number S 839 3 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Purification Protein G Concentration 0. 05mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 0. 1% BSA, 0. 01% Proclin