Human CFTR ELISA Kit Tag Antibody Flow Cytometry Analysis: Load the

Flow Cytometry Analysis: Load the samples onto the flow cytometer for detection

the tissue was removed on the ultra-clean table and placed in the culture dish

GMCSF Immunogen Recombinant Protein Location Secreted Accession P04141 Clone Number S-725-327 Antibody Type Recombinant mAb Isotype IgG Application WB

k Application FCM Reactivity Ms Positive Sample C57BL/6 mouse splenocytes Purification Protein A Concentration 0

Human CFTR ELISA Kit Tag Antibody Flow Cytometry Analysis: Load theProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and